动物造模,关节损伤 z-bio 2022-09-30 603

　　Objective To observe the protective effect of atorvastatin on joint injury in ApoE-/- mice.

　　Methods Forty ApoE-/- mice were randomly divided into a model group and a drug group. They were fed with high-fat diet. The drug group was given atorvastatin 10 mg/(kg·d) by gavage, and the model group was given the same amount of normal saline. Stomach. 20 control group C57BL/6J mice were fed with normal chow. Blood lipid changes were detected by automatic biochemical analyzer. Serum L-6 and TNF-α levels were detected by ELISA. Knee joint tissue samples were taken for HE staining and safranin O-fast green staining to observe histomorphological changes. Transmission electron microscopy was used to observe chondrocyte hyperplasia. Microstructural changes.

　　Results Compared with the model group, the serum TC, TG and LDL-C in the atorvastatin intervention drug group were significantly decreased (P<0.01), while the HDL-C level was increased (P<0.01), and the serum inflammatory factor IIIL-6 and TNF-α decreased significantly (P<0.01), the damage of chondrocyte ultrastructure was significantly improved, the infiltration of inflammatory cells in knee joint synovium was reduced, the damage of cartilage tissue structure and the degree of ossification were significantly reduced, and the disease process was significantly delayed.

　　Conclusion Atorvastatin can protect the joint injury of ApoE-/- mice by regulating blood lipids and down-regulating inflammatory factors.