动物造模 z-bio 2022-03-24 560

　　Objective To explore the differences in physiological and biochemical indicators of different lipids in mice under different feed models, and to provide experimental basis for the selection and evaluation of animal model feed lipids and the use of oils for human health.

　　Methods 120 C57BL/6J male mice were used as the research objects, and two levels were set according to the energy supply of fat, 20% (low oil energy supply level) and 30% (high oil energy supply level, the recommended ratio of daily dietary fat energy supply is not suitable. over 30%), lard (LA), rapeseed oil (RA), olive oil (OL) and blended oil of lard and rapeseed oil (mass ratio 1:1) were selected for each oil energy supply level ( BO). After 16 weeks of feeding, the effects on growth performance, fat deposition, serum biochemical indexes and blood sugar of mice were analyzed.

　　Results Under the low lipid energy supply level, the end body weight of mice in OL group was significantly reduced, the accumulation of perirenal fat and epididymal fat was significantly reduced (P < 0.01), and the Lee's index was also the lowest. The body weight, perirenal fat and epididymal fat accumulation of mice in OL group and RA group were significantly reduced under high lipid energy supply level (P < 0.01). The OL group significantly increased the liver index (P < 0. 01) at both levels of lipid energy supply, while RA significantly increased the renal index (P < 0. 05) at the low lipid energy supply level. Under the energy level, LA and RA groups all significantly increased the renal index (P < 0.01). Under the high lipid energy supply level, the serum TC content of the RA group was significantly lower than that of the other groups (P < 0.05), and the OL group had the lowest TG content. The ratio of HDL-C/LDL-C in the lower RA group was significantly higher than that in the other three groups (P < 0. 05), and there was no difference between the OL group and the OL group under the high lipid energy supply level (P > 0. 05). From the GTT and ITT experiments, BO and OL had better regulation of blood glucose homeostasis under the low lipid energy supply level, and there was no difference between the four groups under the high lipid energy supply level (P > 0.05). Although the blood glucose at the end of the BO group was slightly increased, the overall blood glucose value was lower, which did not cause disorder of blood glucose regulation.

　　Conclusion The 1:1 blended oil of lard and lard oil can increase the body weight and fat deposition in mice under the high oil energy supply level. At the two oil energy supply levels, olive oil reduces body weight and reduces fat deposition, but it may be Adverse effects on liver function, rapeseed oil improved blood lipids best, but it increased the renal index, four kinds of lipids did not damage the ability of mice to regulate blood sugar.