动物实验 z-bo 2020-08-11 537

　　(1) Reproduction method Take adult experimental rhesus monkeys weighing 2 to 5 kg, and they are screened by HBsAg, anti-HAV and other liver function enzyme activities before the experiment; after ketamine anesthesia, blood samples and liver tissue biopsy specimens are collected first, and then It is intravenously inoculated with 0.25-1.0ml experimental sample (the experimental sample virus is the fecal strain and fecal suspension of patients with acute hepatitis A). 4 weeks before the white experiment to 1 week after the experiment, 8-12 weeks (individually 16 weeks), blood was collected on an empty stomach every week for anti-HAV, ALT and ICD detection. Liver biopsy was performed at an interval of 2 to 4 weeks. After modeling, the animals were killed by over-anaesthesia for autopsy and liver histopathological examination.

　　(2) Characteristics of the model: All experimental monkeys developed anti-HAV positive transformation at 2-6 weeks after vaccination, and the antibody titer generally reached a peak at 8-12 weeks. Most rhesus monkeys have fluctuated serum enzyme levels after HAV infection, and they generally have abnormally increased for more than 2 weeks. More than 90% of experimental monkeys can excrete HAV intermittently in the feces within 1 to 3 weeks after infection, and the amount of toxin excretion is relatively large. The high frequency of fecal detoxification indicates that HAV has an active proliferation process in the model monkey, and can induce the body to produce cellular and humoral immune responses. The abnormal findings of serum enzymes ALT, ICD, LDH5 and liver histopathology indicate that the target organs of HAV are damaged.

　　(3) Comparative medicine During the model making process of all experimental monkeys, clinical observations did not find any abnormal clinical manifestations such as listlessness, loss of appetite, jaundice, and no gross pathological changes were found in the autopsy. After the experimental rhesus monkeys were infected with the HAV field strain, the anti-HAV, ALT, ICD, and LDH5 tests were similar to the test results provided by the human hepatitis A course, indicating that the rhesus monkeys can be used as an animal model for human HAV research.