动物造模,肠道菌群 z-bio 2023-05-08 924

　　Objective To study the effect of different concentrations of tea polyphenols on the intestinal flora of mice.

　　Methods Twenty-four C57BL/6 mice were selected and randomly divided into 4 groups: control group (CK, without tea polyphenols), low-dose tea polyphenols group (LTP, with 0.4g/L GTP added in drinking water), medium-dose Tea polyphenol group (MTP, 0.8 g/L GTP added to drinking water), high-dose tea polyphenol group (TP, 1.6 g/L GTP added to drinking water), all four groups were given regular feed, and tea polyphenol drinking water was changed once a day , for 14 consecutive days. Murine gut microbial diversity was analyzed by MiSeq sequencing and bioinformatics.

　　Results High-concentration tea polyphenol intake increased the diversity of intestinal flora. The content of Lactobacillus in the middle-dose and high-dose groups was significantly higher than that in the low-dose and control groups (p=0.038). The proliferative effect of Bacillus is the most obvious and dose-dependent.

　　Conclusion Tea polyphenols can increase the diversity of gut microbes, increase the proportion of probiotics and change the composition of flora.