动物造模,脑缺血/再灌注损伤 z-bio 2022-09-13 466

　　Objective: To investigate the protective effect of stilbene glycoside (TSG) inhibiting NADPH oxidase on cerebral ischemia-reperfusion (I/R) injury in mice.

　　Methods: 100 experimental mice were randomly divided into 5 groups: sham operation group, model group, TSG low-dose group (3 mg/kg), TSG medium-dose group (6 mg/kg), TSG high-dose group (12 mg/kg), There were 20 mice in each group. The cerebral ischemia of mice was induced by bilateral common carotid artery ligation for 2 hours, and the mice were sacrificed after 24 hours of reperfusion. HE staining was used for pathological examination of mouse brain tissue, and DHE staining and ESR spectrometer were used for pathological examination. The levels of reactive oxygen species (ROS) in brain tissue were detected, and the expressions of NOX4 and cleaved caspase-3/9 proteins in brain tissue were detected by Western blot.

　　Results: After cerebral ischemia-reperfusion in mice, the cortical brain tissue in the ischemic area suffered severe pathological damage, the level of ROS was significantly increased, the expression of NOX4 protein in the brain tissue was significantly up-regulated, and the apoptosis-related protein cleaved caspase-3/9 was significantly increased. The protein expression level was significantly increased. TSG can significantly reduce the pathological damage of mouse ischemia-reperfusion brain tissue, inhibit the production of ROS, significantly down-regulate the expression of the oxidative stress protein NOX4, and significantly inhibit the apoptosis-related protein cleavedcaspase-3/9 protein expression.

　　Conclusion: TSG can inhibit the expression of oxidative stress protein NOX4 in brain tissue, reduce the generation of reactive oxygen species, and inhibit the overexpression of apoptosis-related protein cleaved caspase-3/9, thus protecting mice against cerebral ischemia-reperfusion injury.