动物造模 z-bio 2023-02-02 737

　　OBJECTIVE: To encapsulate Nile Red into nano-formulations, and use its fluorescence properties to investigate the tumor targeting and tissue distribution of nano-formulations in tumor-bearing mice.

　　Methods: The cytotoxicity of Nile red nanoemulsion [NRNE(O)] on H1688 was detected by CCK-8 method. A BALB/c nude mouse model of lung cancer was established, and Nile Red Suspension (NRS) and NRNE(O) were administered by gavage. ), and the dynamic distribution of its fluorescence intensity in tumor-bearing nude mice was observed by a small animal in vivo imaging system.

　　RESULTS: NRNE(O) had lower cytotoxicity and good biocompatibility, and its absorption in vivo and accumulation in tumor sites were higher than those of NRS; NR could be rapidly, stably and clearly reflected in nanoformulations Dynamic distribution of nanoformulations in mice.

　　Conclusion: The NRNE(O) prepared in this study has good stability, which provides an important research method for the application of Nile red as a tracer in the study of nano-formulations in animals.