动物造模,巨噬细胞 z-bio 2022-09-21 659

　　Objective: MMP12, also known as macrophage elastase, can almost decompose various extracellular matrix components and vascular components, and is related to the recruitment of monocytes. It was also found that MMP12 is involved in the invasion and metastasis of tumor cells. In addition, MMP12 is also closely related to adipose tissue. The purpose of this study is Changes in macrophages in blood and white fat of MMP12 knockout (MMP12-/-) mice

　　Methods: MMP12-/- mice were genotyped by PCR, and the blood routine of the two groups of mice was detected and analyzed by using littermate MMP12+/+ male mice as controls. Further, flow cytometry was used to detect macrophage markers. Changes of macrophages Finally, the changes of macrophages in adipose tissue after MMP12 deletion were observed by immunohistochemistry and other methods

　　Results: (1) Identification of MMP12-/- mice genotype and expansion (2) Red blood cell count, hemoglobin amount, platelet count, monocyte count in MMP12-/- male mice compared with littermate MMP12+/+ male mice The number of cells and its percentage decreased significantly (P<0.05). The percentage of lymphocytes and eosinophils increased significantly (P<0.05). There was no statistical difference in the absolute count ( 3) The results of flow cytometry indicated that: The ratio of CD11b, a specific marker of phagocytes, to F4/80 double-positive cells was significantly decreased in the blood, and its absolute count was also decreased (4) The results of H&E staining and immunohistochemistry showed that compared with littermate MMP12+/+ male mice CD68 expression was significantly increased in white adipose tissue of MMP12-/- male mice

　　Conclusion: The changes of macrophages may be related to the knockout of MMP12. The significance lies in the discovery that MMP12 may regulate the differentiation of macrophages. The number of macrophages in blood decreases and the number of macrophages in white fat increases.