动物造模,局灶性脑缺血再灌注 z-bio 2022-02-16 454

　　Objective: To study the effect of morroniside on angiogenesis-related factors and neurological function 24 hours after focal cerebral ischemia-reperfusion in rats.

　　METHODS: Healthy adult male SD rats were used to establish focal cerebral ischemia-reperfusion model by suture method, and were randomly divided into sham operation group and model group after operation. 90 mg/kg) and high (270 mg/kg) dose groups were given morroniside 24 hours after operation, once a day for 7 days. The neurobehavioral score, cerebral infarction volume percentage, and cortical angiogenesis were detected. The expression of related proteins was investigated to study the effect of morroniside administration on angiogenesis-related proteins and neurological function recovery 24 h after focal cerebral ischemia-reperfusion in rats.

　　Results: Compared with the sham operation group, the neurological function score and the percentage of cerebral infarction volume in the model group were significantly increased (P<0.001). After 7 days of administration, the high-dose morroniside group significantly decreased the neurological function score compared with the model group. (P<0.01), and could significantly reduce the volume of cerebral infarction (P<0.05). Compared with the sham operation, the expressions of cortical angiogenesis-related proteins CD34 and Ang-1 were increased (P<0.0 P<0.05). Compared with the model Compared with the control group, the expressions of cortical angiogenesis-related proteins CD34 and Ang-1 in the high-dose morroniside group were significantly increased (P<0.0 P<0.01), and the middle-dose group of morroniside could also promote the expression of Ang-1 (P <0.05).

　　Conclusion: Morroniside administration time window is extended to 24h after focal cerebral ischemia-reperfusion, and its high-dose group can reduce neurological function scores, reduce cerebral infarction volume, and regulate CD34 and Ang-1 angiogenesis-related proteins. effect.