动物造模 z-bio 2022-04-25 368

　　Objective: To investigate the intervention effect and mechanism of low molecular weight heparin calcium on mouse embryo implantation.

　　Methods: A mouse pregnancy model was constructed, and LMWHC was injected intraperitoneally for intervention to observe the effect of LMWHC on mouse embryo implantation. Mouse trophoblast cells were isolated and cultured, and the cells were intervened with different concentrations of LMWHC. Transwell chamber was used to detect cell invasiveness, and ELISA and ELISA Western blot and RT-PCR were used to detect the protein and mRNA levels of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-TIMP-2 in the cell supernatant.

　　Results: After intraperitoneal injection of LMWHC in pregnant mice, the number of embryos implanted in pregnant D6 and D9 mice increased significantly (P<0.05). After the concentration of LMWHC intervened in trophoblast cells for 24 h, the number of invasive cells in trophoblast cells were (84.83±14.15), (162.48±25.49), and (98.73±12.77), respectively, compared with (65.27±13.92) cells without LMWHC intervention. ), the difference was significant (P<0.05), the expression level of MMP-2 in the supernatant of trophoblast cells was higher than that in the cells without LMWHC intervention (P<0.05), and the expression level of TIMP-2 in the supernatant of trophoblast cells was higher than that in the cells without LMWHC intervention (P<0.05). The expression level of -2 was lower than that in cells without LMWHC intervention (P<0.05).

　　Conclusion: LMWHC can promote the implantation of mouse embryos, and the mechanism may be related to the enhancement of trophoblast cell invasiveness by LMWHC by affecting the expression of MMP-2 and TIMP-2.