动物造模,慢性萎缩性胃炎 z-bio 2023-01-12 378

　　Objective: To investigate the effect and molecular mechanism of Dendrobium officinale polysaccharide on nitrosoguanidine-induced chronic atrophic gastritis in rats.

　　Methods: 120 rats were randomly divided into normal group, model group, Yangweishu group, Dendrobium candidum polysaccharide high, medium and low dose groups, 20 in each group. Model group, Yangweishu group, Dendrobium candidum polysaccharide high and medium ､The low-dose group was given 167 μg/mL MNNG drinking liquid, and the normal group was given free access to distilled water for a total of 8 weeks of modeling. After successful modeling, the high-dose Dendrobium officinale polysaccharide group was given 1.34 g/kg of Dendrobium officinale polysaccharide extract by gavage daily. ; middle dose group 0.67g/kg; low dose group 0.335g/kg. Yangweishu group was given Yangweishu granules 4.0g/kg by gavage every day. The model group was given normal saline by gavage, and the normal group was not intervened. After 12 weeks of treatment, gastric tissue was taken out for histopathology, gastrin (Gas), interleukin-6 (IL-6), proliferating cell nuclear antigen (PCNA), tumor necrosis factor-α (tumornecrosis factor- α, TNF-α mRNA), Janus kinase 2 (JAK2), activator-3/tyrosine phosphorylation 3 (p-STAT3/STAT3). Results After the 12th week of treatment, the normal group had higher levels of Dendrobium officinale polysaccharide. The body weights of the middle and low dose groups and the Yangweishu group were higher than those of the model group (P<0.05). The body weight of the Dendrobium officinale polysaccharide high and middle dose groups were higher than those of the Yangweishu group (P<0.05); ② the inflammation grade of the model group higher than the normal group (P<0.01), while the number of glands, gland thickness, chief cells, and parietal cells were lower than those in the model group (P<0.05). The inflammation grade, number of glands, thickness of glands, number of chief cells, and number of parietal cells in the group were all recovered compared with those in the model group (P<0.05). The number of parietal cells increased (P<0.05); the number of parietal cells in the high-dose Dendrobium officinale polysaccharide group increased (P<0.05); ③ In the normal group, the Dendrobium officinale polysaccharide high, medium and low dose groups and the Yangweishu group had higher serum GAS levels than Model group (P<0.05). In the normal group, the serum IL-6 content of the high-dose D. officinale polysaccharide group was lower than that of the model group (P<0.05); ④ the normal group, the PCNA and TNF- αmRNA was lower than that in the model group (P<0.05); ⑤In the normal group, the protein expression of Dendrobium officinale polysaccharide high and medium dose groups JAK2 and p-STAT3/STAT3 were lower than those in the model group (P<0.05). Conclusion Rats have good protection and reversal effects, can effectively restore body weight and relieve gastric mucosal atrophy, and its mechanism of action may be to improve the pathological state of gastric mucosa by inhibiting the activation of JAK2/p-STAT3 signaling pathway.