动物造模,非肥胖型糖尿病 z-bio 2022-10-07 477

　　Objective: To improve the effect of in vitro fertilization in mice with non-obese diabetic background.

　　Methods: The experiments were carried out using NOD background female mice of different ages. The experiment was divided into four groups, 4, 6, 8 and 10 weeks old female mice, respectively, and 3-6 month old NOD male mice were subjected to in vitro fertilization, and the next day The number of embryos available for each female mouse was counted, and the experiment was repeated twice. The data of NOD mice transplanted from 2016 to 2018 were collected for comparison.

　　Results: In the first experiment, the number of available two-cell embryos after in vitro fertilization in the four groups of 4, 6, 8 and 10 weeks was (13.4±2.5) and (22.7±2.9), respectively. ), (22.0±7.0), and (30.5±10.9). In the second experiment, the number of available two-cell embryos for each female mouse in groups 4, 6, 8 and 10 weeks of age was expressed as the mean ± standard deviation, respectively. (7.8 ± 2.6), (16.5 ± 4.5), (14.2 ± 6.5), (24.5 ± 5.9). 4, 8, 10-week-old female mice after IVF embryo transfer mean birth rate ± standard deviation They were (35.32±9.9)%, (36.19±6.2)%, and (43.15±6.9)% respectively.

　　Conclusion: 10-week-old female mice with NOD background have the best in vitro fertilization effect, and it is recommended to use 10-week-old female mice for in vitro fertilization.