动物造模,多囊卵巢综合征 z-bio 2022-04-27 298

　　Objective: To observe the expression changes of DR5 and DcR2 in ovarian granulosa cells of rats with polycystic ovary syndrome, and to explore the possible mechanism of follicular developmental disorders in rats with PCOS.

　　Methods: Rats were injected subcutaneously with pratestosterone sulfate to construct PCOS model. Immunohistochemistry and RT-qPCR were used to observe and compare the expressions of DR5 and DcR2 in ovarian granulosa cells of PCOS group and control group.

　　Results: Immunohistochemical results showed that the expression of DR5 in ovarian granulosa cells of the PCOS group was significantly enhanced compared with the control group (P<0.05). Compared with the control group, the expression of DcR2 was significantly enhanced in the follicles of the same grade (P<0.05), and="" the="" expression="" in="" primordial="" follicle="" granulosa="" cells="" had="" no="" significant="" difference="" p="">0.05). RT-qPCR analysis showed that DR5mRNA and DcR2mRNA were significantly higher in the two groups. Both were expressed in PCOS group, and the expression of both in ovarian granulosa cells of PCOS group was significantly higher than that of normal control group (P<0.05).

　　Conclusion: The abnormal expression of DR5 and DcR2 in granulosa cells may play a certain role in the abnormal development of follicles in PCOS rats, and they may have a certain impact on the development of follicles by participating in the apoptosis of granulosa cells.