动物造模,糖尿病 z-bio 2022-02-17 311

　　OBJECTIVE: To study the changes of myocardial fibrosis and myocardial AGEs/RAGE levels by constructing a type Ⅱ diabetic rat model.

　　Methods: Male SD rats were fed with high-fat diet for 6 weeks, then fasted overnight for 12 h, and injected intraperitoneally with low-dose streptozotocin (STZ, 30 mg/kg). 3 and 7 days after the injection, the random blood glucose concentration of the rats was detected, twice. Random blood glucose ≥ 16.7 mmol/L indicates that the model of type Ⅱ diabetic rats is successful. At 4, 8 and 12 weeks after the successful modeling, the blood FBG, insulin and left ventricular AGEs, Hyp content, Masson staining and RAGE expression were detected. The change.

　　Results: At the 4th, 8th and 12th weeks after the successful modeling, the blood FBG level of the diabetic control group (4D, 8D, 12D) was significantly higher than that of the normal control group (4C, 8C, 12C) in the same week, and the body weight was significantly decreased; At the 12th week after the successful modeling, the insulin in the diabetic control group (12D) was significantly higher than that in the normal control group (12C) and the diabetic control group (4D) at the 4th week; from the 8th week after the successful modeling Compared with normal control group (8C, 12C), myocardial Hyp, CVF, AGEs and RAGE of diabetic control group (8D, 12D) were significantly increased; the level of myocardial AGEs/RAGE in type 2 diabetic rats was significantly positively correlated with CVF.

　　Conclusion: High-fat diet feeding and intraperitoneal injection of low-dose STZ can successfully replicate the type II diabetic rat model. With the prolongation of the disease course, myocardial fibrosis, myocardial AGEs content and RAGE protein expression all showed a gradual upward trend, and type II diabetes There is a positive correlation between myocardial AGEs/RAGE levels and myocardial fibrosis in diabetic rats.