动物造模 z-bio 2022-11-29 357

　　OBJECTIVE: To study the protective effect of 13-methyltetradecanoic acid on abnormal oxygen in rat cerebral cortical astrocytes.

　　Methods: The primary cerebral cortical astrocytes of neonatal SD neonatal rats were subcultured, and the oxygen-glucose deprivation/reoxygenation glucose (OGD/R) method was used to replicate the model of abnormal oxygen. Interventions with 13-MTD20, 40, 80 μg/mL (M20, M40, M80) were given, the morphology of astrocytes was dynamically observed by inverted microscope, glial fibrillary acidic protein (GFAP) was identified by cellular immunochemistry, and mitochondrial activity was detected by MTT method. The expression of aquaporin 4 (AQP4) protein in astrocytes was detected by immunohistochemistry.

　　Results: After OGD10h/R24h injury, SD rat cerebral cortical astrocytes were significantly damaged, mitochondrial activity was significantly decreased (P<0.01), and the expression of AQP4 protein in astrocyte membrane was significantly increased (P<0.01). ); compared with the model group, 13-MTD20, 40, and 80 μg/mL could reduce damage, increase mitochondrial activity, and reduce AQP4 protein expression, and 80 μg/mL had the best effect (P<0.01).

　　Conclusion: 13-MTD can reduce the expression of AQP4, increase mitochondrial activity, reduce cell edema, and then protect astrocyte damage induced by abnormal oxygen.