动物造模 z-bio 2022-10-22 476

　　OBJECTIVE: To explore the effect of androgen deficiency on the effects of androgen deficiency on high-fat and high-cholesterol diets by analyzing the liver expression profiles of non-castrated (SHAM), castrated (CAS) and castrated+testosterone-treated (CAS+T) minipigs fed high-fat and high-cholesterol diets. Effects of feeding minipig liver mRNA alternative splicing.

　　METHODS: RNA-Seq technology was used to sequence the expression profiles of SHAM, CAS and CAS+T minipig livers. TopHat software was used to screen the differential AS genes in the three groups of minipig livers, and the common genes in the three groups of samples were analyzed. Differential AS genes were subjected to gene annotation and metabolic pathway enrichment analysis.

　　Results: (1) Castration and testosterone treatment can induce changes in mRNA alternative splicing in high-fat and high-cholesterol-fed minipig livers; (2) There are 113 shared differential AS genes between SHAMvs.CAS and CAS+Tvs.CAS, These include genes related to glycolipid metabolism such as AGPAT6, NR1H4, PPARD and GK; (3) GO and KEGG analysis results: the above 113 differential AS genes are mainly involved in fatty acid metabolism, glyceride metabolism, glucose response and adipocytokine signaling, etc. biological processes and pathways.

　　Conclusion: Androgen deficiency can lead to altered mRNA alternative splicing in the liver of high-fat and high-cholesterol-fed minipigs, and may affect the pathogenesis of high-fat-induced fatty liver by regulating hepatic glucose and lipid metabolism.