动物造模,急性肺损伤 z-bio 2022-06-13 666

　　Objective: To investigate the effect of liensinine on lipopolysaccharide-induced acute lung injury (ALI) in mice.

　　Methods: BALB/c mice were randomly divided into control group, LPS group, LPS + Liensinine (2, 4, 8 mg/kg) group, and dexamethasone (5 mg/kg) group. model of acute lung injury. After 12 hours, the pathological changes of lung tissue were observed by histology; the contents of TNF-α, IL-6 and IL-1β in bronchoalveolar lavage fluid (BALF) were detected by ELISA; the number of neutrophils in BALF was detected by Wright-Giemsa staining ; BCA method to detect total protein content; Evans blue to detect pulmonary capillary permeability; spectrophotometry to detect MPO activity, MDA content, SOD activity and GSH content in lung homogenate supernatant; flow cytometry to detect lung ROS content in tissues.

　　Results: LPS group showed inflammatory cell infiltration, bronchoalveolar wall thickening and pulmonary congestion in lung tissue, while liensinine group could improve lung injury; the contents of TNF-α, IL-6 and IL-1β in BALF of LPS group significantly increased neutrophil count and total protein content, increased pulmonary capillary permeability, MPO activity and MDA content, decreased SOD activity and GSH content, and increased ROS content, while the liensinine group can reduce TNF-α in BALF. α, IL-6, IL-1β content, decreased neutrophil count and total protein content, decreased pulmonary capillary permeability, decreased MPO activity and MDA content, increased SOD activity and GSH content, and decreased ROS content.

　　Conclusion: Liensinine can protect LPS-induced acute lung injury in mice through anti-inflammatory and antioxidant effects.