动物造模,造血干细胞 z-bio 2022-11-10 485

　　OBJECTIVE: To examine the effects of different administration methods on the function of hematopoietic stem cells in mice.

　　Methods: C57 mice, intraperitoneal administration of busulfan, were divided into high-dose group (40 mg/kg) and low-dose group (20 mg/kg). The low-dose group was administered once, and the high-dose group was administered for two days. Administered at 20 mg/kg per day. The peripheral blood and bone marrow cell counts, hematopoietic stem cells, hematopoietic progenitor cells and long-term hematopoietic stem cells were detected 15 d and 30 d after administration, respectively, and the proliferation ability of mouse hematopoietic progenitor cells was evaluated by CFU-GM experiment. Hematopoietic stem cells were cultured in vitro, and the function of hematopoietic stem cells was detected by single-cell colony assay.

　　Results: Both high-dose and low-dose groups of busulfan could reduce the number of leukocytes and platelets in the peripheral blood of C57 mice, reduce the proportion of hematopoietic stem and progenitor cells and long-term hematopoietic stem cells, and reduce the ability of CFU-GM and single cell colony formation. The injury in the high-dose group was further aggravated than that in the low-dose group? There was no significant difference in the body weight of the mice compared with the control group.

　　Conclusion: Busulfan in high-dose group can induce hematopoietic stem cell injury after 15 days of administration, which provides a model and basis for the study of hematopoietic stem cell injury mechanism and injury protection.