动物造模,脊髓损伤 z-bio 2022-09-26 451

　　Objective: To investigate the effect of artificial recombinant botulinum toxin type A heavy chain (BoNT/A heavy chain) on the expression of local growth-related proteins in rats with spinal cord injury in vivo, and to provide a basis for elucidating the mechanism of BoNT/A heavy chain promoting neurite regeneration.

　　Methods: A rat model of unilateral lumbar spinal cord transection injury was established, and BoNT/A heavy chain was administered locally and intrathecally; two-dimensional electrophoresis was performed on the local bone marrow tissue at different times after administration to detect the overall protein expression; 43) and superior cervical ganglion protein 10 (SCG 10) were detected by western blotting and immunofluorescence to observe their expression and distribution under the influence of BoNT/A heavy chain.

　　Results: (1) The model animals with unilateral lumbar spinal cord amputation showed obvious unilateral motor and sensory dysfunction; (2) The administration of BoNT/A heavy chain after spinal cord injury could affect the changes in the local protein expression profile of the injury: BoNT/A heavy chain It can cause the damage local protein point group to reverse or further increase the expression on the basis of the change, especially the MW is located in 35 ~ 45 kDa and 18 ~ 25 kDa. The protein spots with isoelectric points in the range of 5 to 7 were obvious; (3) Western blotting and immunofluorescence staining showed that: BoNT/A heavy chain could promote the expression of p-GAP 43 and SCG 10 (P<0.05), and p -GAP 43 and SCG 10 were mainly positive in the cells around the injury, and both cytoplasm and processes were positive.

　　Conclusion: Administration of BoNT/A heavy chain after spinal cord injury can promote the expression of selective growth-related proteins p-GAP 43 and SCG 10 after spinal cord injury.