动物造模,2型糖尿病肾病 z-bio 2022-02-18 428

　　Objective: To explore the effects of different high-fat diet feeding time on blood glucose, insulin resistance index (HOMA-IR) and urinary albumin excretion rate (UAER) in induced type 2 diabetic nephropathy (DN) rat model.

　　Methods: The model was established by the method of unilateral renal artery ligation + high-fat diet feeding + low-dose streptozotocin (STZ) intraperitoneal injection. After ligating the unilateral renal artery, DN1 group and DN2 group were respectively fed high-fat diet for 4 days. After 4 weeks of STZ injection and at the end of the experiment, the UAER of the two groups of rats was compared, and the blood glucose, body weight, HOMA-IR, renal index and renal index of the two groups of rats were observed. Kidney pathology, etc.?

　　Results: Four weeks after STZ injection, UAER increased in both groups, and the UAER in DN2 group was significantly higher than that in DN1 group (P<0.01). At the end of the 12th week, the HE staining of kidney tissue in both DN1 and DN2 groups could be seen Hypertrophy of glomerular capillary loops, increased mesangial matrix; renal small cyst stenosis and other pathological changes? The body weight of the DN2 group was higher than that of the DN1 group ( P < 0.01), the renal index was lower than that of the DN1 group ( P < 0.01), and the blood glucose? Serum insulin levels? HOMA-IR and UAER had no significant difference compared with DN1 group ( P >0.05)?

　　Conclusion: Prolonged feeding time of high-fat diet can aggravate renal damage in DN, but it may delay the intervention time, so the choice should be weighed in the trial?