动物造模 z-bio 2022-03-25 553

　　Objective: To establish a scopolamine memory impairment model, use the traditional Chinese medicine compound Jinsi to intervene, observe the effect of Jinsi on the behavior and cholinergic system of the scopolamine-induced memory impairment model in mice, and explore the neuroprotective mechanism of the drug?

　　Methods: ICR mice were randomly divided into normal control group; model group, vehicle 0.5% CMC; positive control group, donepezil, 0.92 mg/(kg·d); 5 mg/(kg·d)? 18 mice in each group, 0.1 mL/10 g of mice body weight were given by intragastric administration for 30 d? Scopolamine 3 mg/(kg·d), dissolved in 0.9% normal saline, injected at 0.1 mL/10 g body weight of mice, to carry out Morris water maze test? After the experiment, cortical and hippocampal tissues were collected to measure acetylcholine in cortex and hippocampus respectively (Ach) content? Acetylcholinesterase (AchE) and choline acetyltransferase (ChAT) activity?

　　RESULTS: Jin Siwei could shorten the swimming distance and swimming time of the model mice and increase the staying time of the target quadrant; Jin Siwei could increase the Ach content in the brain of the model mice? The AchE activity decreased and the ChAT activity increased?

　　Conclusion: Jin Siwei can improve the learning and memory ability of scopolamine-induced memory impairment model mice, and the mechanism may be related to the cholinergic system?