动物造模 z-bio 2022-02-18 572

　　Objective: To explore the effect and possible mechanism of metformin supplementation on the learning and memory ability of mice during the aging process of D-galactose-induced mice.

　　Methods: Twenty-four female ICR mice were randomly divided into 3 groups: control group, aging group, and aging + metformin group, with 8 mice in each group, for 16 weeks of continuous administration. The body weight and food intake of the mice in each group were monitored; the learning and memory ability of the mice was tested by behavior; the structure of the hippocampus of the mice was observed by HE staining; the level of glutathione (GSH) in the hippocampus of the mice in each group was detected by colorimetry.

　　Results: Compared with the aging group, the weight of mice in the aging + metformin group decreased (P < 0.05); the escape latency and swimming distance of the Morris water maze were significantly reduced (P < 0.01 or P < 0.05), and the swimming time in the target quadrant was prolonged (P < 0.05). < 0.05), the swimming speed increased (P < 0.05); the number of active avoidance in the shuttle experiment increased (P < 0.05); HE staining showed that the nucleus shrinkage and hyperchromatic hippocampal neurons in the dentate gyrus of the hippocampus were significantly reduced; GSH in the hippocampus levels were significantly increased (P < 0.05).

　　Conclusion: Metformin supplementation can significantly delay the decline of learning and memory ability in mice during the aging process, and maintain the normal structure of hippocampal neurons. The mechanism may be related to reducing the body weight of mice and enhancing the antioxidant level of hippocampal tissue.