动物造模,神经炎性损伤 z-bio 2023-01-13 1116

　　Objective: To detect the expression of TRPC6 channel in microglia in a mouse MPTP-induced neuroinflammation model, and to explore the effect of TRPC6 channel on inflammatory expression and dopaminergic neuron damage.

　　Methods: In MPTP-injected mice, microglia in the substantia nigra region were sorted by magnetic beads labeled with CD11b, and the expression of TRPC6 was detected by western blotting. After constructing CD11b-TRPC6-/- mice, MPTP-induced proliferation of microglia and damage of dopaminergic neurons were detected by immunofluorescence. Combined with western blotting and real-time quantitative PCR, the protein level of cryαB and the expression of inflammatory factors were detected after TRPC6 knockout in MPTP model.

　　RESULTS: The expression of TRPC6 channels in microglia was significantly up-regulated in MPTP-injected mice. In addition, the level of cryαB protein in microglia was up-regulated in CD11b-TRPC6-/- mice injected with MPTP. At the same time, MPTP-induced proliferation of microglia and enhanced expression of inflammatory factors were inhibited, while the damage of dopaminergic neurons was alleviated.

　　Conclusion: The expression of TRPC6 channel in microglia is up-regulated in MPTP model, and knockout of TRPC6 can increase the level of cryαB protein in microglia and reduce the expression of inflammatory factors, thereby reducing the damage of dopaminergic neurons.