动物模型,肾功能衰竭 z-bo 2020-08-12 504

　　1. Chronic renal failure caused by freezing in rats

　　[Operation steps] 200～300g male rats (measured without proteinuria before surgery), after anesthesia, incision on the left side of the abdomen, expose the left side, peel off the renal fascia, put a cold knife (1.2cm) pre-dipped in a liquid nitrogen bottle ×0.8cm×15cm), in the upper and lower poles and lateral front and rear of the kidney in sequence, frozen for 40s each time, and then reset the kidney to end the operation. Two weeks later, the right kidney was surgically removed. After the operation, it was placed in a metabolic cage and showed stable chronic renal failure for 6-12 weeks, and it was available for experimentation from the 8th week.

　　[Result analysis] Observation indicators include: body weight, survival rate, blood creatinine, serum total protein and albumin, serum urea nitrogen and renal pathology examination.

　　2. Chronic renal failure caused by partial nephrectomy in rats

　　[Operation procedure] After anesthesia, a male rat weighing about 200g (no proteinuria was measured before the operation), after anesthesia, make a midline incision in the abdomen to expose the right kidney, peel off the renal fascia, ligate the kidney hilum, remove the right kidney, and retain the adrenal gland. Then the left kidney is exposed, the kidney parenchyma of the upper and lower poles is quickly removed, and the bleeding is stopped by compression. The abdominal cavity was closed and placed in a metabolic cage after the operation. The patient showed stable chronic renal failure from 14 to 16 weeks and was available for experimentation at the 14th week.

　　[Result analysis] Observation indicators include: body weight, survival rate, blood creatinine, serum total protein and albumin, serum urea nitrogen and renal pathology examination.