动物模型,疱疹病毒 z-bo 2020-08-12 481

　　(1) Replication method The neural stellate cell-specific expression vector containing the human herpes virus 6BU83 gene was constructed, and after microinjection into mouse fertilized egg cells, a transgenic mouse line expressing human herpes virus 6BU83 was established.

　　(2) Features of the model This model is a nucleic acid fragment that is specifically expressed in astrocytes of the mouse central nervous system. The first segment of this fragment is the glial fibrillary acidic protein promoter, which can guide the good specific expression in central nervous system astrocytes. There is a mouse protamine 1 gene fragment at the end, which provides an intron and poly A site. The HHV-6B U83 nucleic acid fragment is inserted in the multiple cloning site between the two. Astrocytes are one of the main cell groups that make up the central nervous system of vertebrates, and are related to a wide range of functions of the central nervous system, such as signal transduction and energy metabolism.

　　(3) Comparative medicine. The establishment of transgenic mice expressing HHV virus protein is of great significance to the study of the relationship between HHV infection and related diseases. Observation of transgenic mice may provide direct and important correlations for some unexplained diseases. in accordance with. The establishment of HHV-specific gene-expressing mice provides a meaningful animal model for further research on the relationship between HHV and diseases, and will help to clarify the pathogenicity of HHV and explore the mechanism of certain diseases with unknown etiology.