动物造模,溃疡性结肠炎,巨噬细胞 z-bio 2022-10-26 654

　　Objective: To investigate the role of macrophage NF-κB activating factor 1 in inflammatory bowel disease.

　　Methods: The mice whose macrophage Act1 expression was inhibited (anti-Act1) were used as the research objects, and the ulcerative colitis model was induced by dextran sodium sulfate (DSS). Disease activity index scores were performed on colorectal tissue, blood in the stool, stool viscosity, etc., and the severity of inflammation was evaluated by HE staining on colorectal tissue. expression changes.

　　Results: Compared with C57 mice after DSS induction for 7 days, anti-Act1 mice had lower scores of colorectal inflammation indexes, less infiltration numbers such as macrophages, and mRNA expressions of TNF-α, IL-1β and IL-6. lower.

　　Conclusion: Targeted inhibition of Act1 expression in macrophages can alleviate DSS-induced ulcerative colitis.