动物造模 z-bio 2022-03-25 434

　　OBJECTIVE: To explore the distribution and differential expression of Hba-α protein in hatched blastocysts and dormant embryos of mice before and after freezing by means of programmed freezing, so as to provide a theoretical basis for the future development and utilization of new mammalian antifreeze proteins .

　　Methods: Hatching blastocysts were obtained from pregnant d5 mice; dormant embryos were obtained from mouse delayed implantation model, and the distribution and expression of Hba-α protein in each group of embryos before and after programmed freezing were analyzed by Confocal microscope and Western Blot technology. detection.

　　Results: Both hatched blastocysts and dormant embryos expressed Hba-α protein before and after programmed freezing; there was no significant difference in Hba-α protein expression between hatched blastocysts after programmed freezing and before freezing (P> Compared with hatched blastocysts before and after programmed freezing, the expression of Hba-α protein in dormant embryos before freezing was significantly down-regulated (P<0.05); the expression of Hba-α protein in dormant embryos after freezing was significantly lower than that in other embryos. group (P<0.05).

　　Conclusion: The effect of programmed freezing on the expression of Hba-α protein in mouse dormant embryos is significantly greater than that in hatched blastocysts; Hba-α gene has the potential as a new mammalian antifreeze protein.