动物造模,急性心肌梗死 z-bio 2022-05-17 724

　　Objective: To study whether ventricular extracellular matrix (ECM) can promote the differentiation of cardiomyocyte-derived cardiac stem cells (CDC) implanted into myocardial tissue of myocardial infarction into cardiomyocytes, vascular endothelial and vascular smooth muscle cells, and improve the effect of myocardial infarction in rats. cardiac structure and function.

　　Methods: CDC was cultured by myocardial tissue block culture method, ECM was prepared by decellularization method, and an acute myocardial infarction model was established by ligating the anterior descending artery of Wistar rats. IMDM (group I), ECM suspension (group E), IMDM solution containing 106CDCs (group IC), and ECM suspension containing 106CDCs (group EC) were injected into the myocardial tissue of myocardial infarction, respectively. only. After 3 weeks, the cardiac function was evaluated by color Doppler ultrasound, the percentage of positive area of myocardial fibrosis was analyzed by Masson staining, and the expression of vWF, α-SA and α-SMA in vivo by CDC was qualitatively analyzed by immunofluorescence.

　　Results: After 3 weeks of myocardial infarction, the left ventricular ejection fraction and short-axis contraction rate (FS%) of the EC group were the highest; the percentage of positive area of myocardial fibrosis after myocardial infarction in the EC group was the lowest; The percentage of differentiation-positive area was higher, P<0.05.

　　Conclusion: Myocardial tissue-derived ECM can promote the differentiation of CDC implanted into myocardial infarction tissue into myocardial, endothelial and smooth muscle cells. Combined transplantation of CDC and ECM can achieve optimal cardiac structural and functional benefits.