动物造模 z-bio 2022-02-25 626

　　Objective: To explore the changes of SOD activity and protein expression in main organs of diabetic rats, and to observe the effect of the antioxidant N-acetylcysteine (NAC) on the antioxidant status of the body after short-term treatment (4 weeks). .

　　Methods: STZ-induced diabetic rats (group D, n=8) were given NAC 1.5 g/kg by gavage every day (group D+N, n=8), and the normal control group (group C, n=8) was given at the same time The same volume of normal saline. After 4 weeks, heart, lung, liver and kidney tissues were obtained, and the kits were used to detect plasma total SOD, total antioxidant concentration, lipid peroxidation specific marker 15-F2t-isoprostane and total superoxide dismutase (SOD) ( superoxide dismutase, SOD) activity, Western blotting analysis of SOD isoforms Cu/Zn-SOD and Mn-SOD protein expression levels.

　　Results: Compared with group C, the concentration of plasma 15-F2t-isoprostane and total antioxidants and the total SOD activity in myocardial tissue of rats in group D were significantly increased, while the total SOD activity in plasma, lung, liver and kidney tissue was significantly decreased; The expression level of Cu/Zn-SOD protein in heart and lung tissue was significantly increased, while that in liver and kidney tissue was significantly decreased. The change is not obvious. NAC intervention can reverse the above changes to varying degrees, but further reduce the expression of Mn-SOD in renal tissue.

　　Conclusion: The total SOD activity, Cu/Zn-SOD and Mn-SOD protein expression levels in various tissues of diabetic rats have tissue differences. Delays the effects of diabetes-related target organ damage.