动物造模 z-bio 2022-02-28 374

　　Objective: Based on the study of the subchronic oral toxicity of diflubenzuron to rats, the effect on the blood system of rats after exposure was preliminarily discussed.

　　Methods: 80 SPF SD rats were randomly divided into 4 groups according to their body weight, 20 rats in each group, half male and half male. Feeds containing 0, 25, 500 and 10000 mg/kg of diflubenzuron were continuously fed and exposed to the poison for 3 months, respectively, and the clinical manifestations, food intake and body weight of the rats in each group were observed. Blood biochemical indexes, organ weights, organ coefficients and histopathological changes.

　　RESULTS: During the experiment, the food, activity and drinking water of the female and male rats in each dose group were basically normal, and no obvious poisoning was found. The blood red blood cell count (RBC), hemoglobin concentration (HGB) and hematocrit (HCT) of the rats in the middle and high dose groups decreased, while the mean corpuscular volume (MCV) and red blood cell distribution width (RDW) of the rats in the high dose group increased. And male mice serum total bilirubin (TBIL) increased, compared with the control group, the difference was statistically significant (P<0.01 or 0.05). The spleen weight and organ coefficient of the rats in the high-dose group were increased, and the liver weight and organ coefficient of the female rats were increased. Compared with the control group, the difference was statistically significant (P<0.01). All the animals in the high-dose group and some animals in the middle-dose group had significantly enlarged spleens, hard texture and darker color. Pathological examination showed that the spleen red pulp sinus was highly dilated and filled with a large number of red blood cells, and the medulla had obvious hemosiderin deposition.

　　Conclusion: Diflubenzuron has obvious damage to the blood system of rats, and long-term exposure may lead to spleen enlargement and chronic hemolytic anemia.