动物造模,迟发性抑郁 z-bio 2022-04-27 436

　　Objective: To investigate the effect of continuous long-term rapid eye movement sleep deprivation (5 d) on depression-like behavior and the level of amygdala monoamine oxidase A in C57BL/6J mice.

　　Methods: Adult male C57BL/6J mice were randomly divided into blank group, control group and REM sleep deprivation group, and a REM sleep deprivation model was established by the multi-small platform water environment method. After sleep deprivation, the open field test was used to test the exercise ability of each group at different time points, and the forced swimming test and sugar water preference test were used to test the depression-like behavior of each group. After that, the amygdala was isolated from the brain, and the expression level of MAO in each group was detected by Western Blot and Real-time PCR.

　　Results: Within 1-3 days after REM sleep deprivation, the exercise ability of sleep-deprived mice was significantly decreased, and returned to normal levels on the 4th day; the forced swimming test on the 7th-14th day showed that the immobility time of the mice was prolonged; sugar water The preference rate is also significantly reduced. Western blot and Real-time PCR detection showed that the expression level of monoamine oxidase A in amygdala was significantly increased.

　　CONCLUSION: Continuous prolonged REM sleep deprivation leads to delayed-onset depression-like behavior in mice, and this phenomenon is likely related to changes in the level of monoamine oxidase in the amygdala.