动物造模 z-bio 2022-02-28 410

　　Objective: To observe the effect of triptolide (TPT) on the expression of Notch receptor and ligand in adjuvant arthritis (AA) rats.

　　Methods: 40 rats were randomly divided into normal control (NC) group, model (MC) group, methotrexate (MTX) group, and TPT group, 10 in each group, and the other animals except the NC group were given right Inflammation was induced by intradermal injection of 0.1 mL of Freund's complete adjuvant on the hind paw, and the AA rat model was replicated. NC group and MC group were given normal saline, and the other groups were given MTX and TPT, respectively. After 30 days of administration, the changes of toe swelling (E), arthritis index (AI), lung function and Notch receptor/ligand were detected in each group.

　　Results: The expressions of E, AI, Notch3, Notch4, and Delta1 in the MC group were significantly increased; the expressions of pulmonary function parameters, Notch1, Jagged1, and Jagged2 were decreased (P<0.01); The expressions of Jagged1 and Jagged2 were increased, and the expressions of E, AI, Notch3, Notch4, and Delta1 were decreased, and the curative effect was better than that of MTX in the control group (P<0.05, P<0.01).

　　Conclusion: TPT may improve the symptoms of AA joints and lungs by up-regulating the expressions of Delta1, Notch3 and Notch4, and down-regulating the expressions of Jagged1, Jagged2 and Notch1, reducing inflammatory response and immune complex deposition.