动物造模 z-bio 2022-03-01 420

　　Objective: To establish a mouse model of mechanical lung injury by ventilator hyperventilation, intervene with angiotensin II receptor inhibitor losartan, and explore the effect of losartan on caveolin-1 in the lung of mice with mechanically ventilated lung injury. and regulation of oxidative stress-related proteins.

　　METHODS: Thirty-six male C57 mice were randomly divided into control group, mechanical ventilation group, simple drug control group and treatment group. After modeling, the acute lung injury and the expression and interaction of related proteins were detected in each group.

　　Results: The Smith score of lung injury in the mechanical ventilation group was 3.3, which was significantly higher than that in the control group (0.4) and the drug control group (0.3). The Smith score in the treatment group was 2.3, which was significantly lower than that in the mechanical ventilation group (P<0.05). The expression of caveolin-1 and NOX4 in the lung tissue of mice in the group was significantly higher than that in the control group and the simple drug control group (P<0.05). The expression of the above proteins in the lung tissue was significantly lower than that in the mechanical ventilation group (P<0.05), and fluorescence co-staining indicated that the overlapping area of caveolin-1 and NOX4 fluorescence was reduced compared with the mechanical ventilation group.

　　Conclusion: Losartan can alleviate the mechanical ventilation-induced lung injury and inhibit the expression and mutual approach of caveolin-1 and NOX4 in a mouse model of acute lung injury induced by mechanical ventilation.