动物造模 z-bio 2022-03-01 399

　　OBJECTIVE: To investigate the effect of telemetry EEG activity in the sublimbic region on drug-seeking behavior in morphine-dependent rats during drug-seeking regression and its mechanism.

　　METHODS: SD rats were randomly divided into a model group and a control group. Stereotaxic electrodes were implanted in the IL area. The rats in the model group were made into morphine dependence and regression models in turn. The wireless telemetry system was used to record the brain in the IL area of the rats during regression. Changes in electrical activity.

　　Results: The white box residence time of rats in the model group for 1-2 days after withdrawal was significantly longer than that before morphine injection and the control group; 1-2 days after extinction training, the white box residence time of rats in the model group was significantly shorter than that of the withdrawal period, which was similar to that of morphine. There was no significant difference between pre-injection and the control group. Compared with the control group, when the model group stayed in the white box for 2 days of withdrawal, the EEG β wave in the IL area increased significantly, and the δ wave decreased significantly; when traveling from the black box to the white box, the δ wave increased significantly, and the α wave and β wave decreased significantly. . After disappearing for 2 days, when the rats in the model group stayed in the white box, the EEG in the IL area was significantly lower than that of the control group. , compared with the control group, there was no significant difference in IL EEG, but compared with the withdrawal period, the delta wave was significantly reduced, and the alpha and beta waves were significantly increased.

　　CONCLUSION: The EEG activity in the IL region of morphine-dependent rats changes abnormally during the drug-seeking extinction period. This abnormal EEG generation may affect the formation of drug-seeking motivation, thereby inhibiting the expression and maintenance of drug-seeking behavior.