动物造模 z-bio 2022-03-02 408

　　Objective: To investigate the therapeutic value and mechanism of CD4+ CD25+ Foxp3+ Treg cells in experimental autoimmune myositis (EAM) mice.

　　METHODS: Immunomagnetic bead sorting technology was used to separate sufficient CD4+ CD25+ Foxp3+ Treg cells from the spleen of BALC/c mice for re-infusion. The pathological changes of EAM mice in the intervention group and the non-intervention group were observed. Flow cytometry The expression changes of PD-1 and CTLA-4 on the surface of spleen CD4+ CD25+ Foxp3+ Treg cells of the two groups of mice were detected by instrument, and the changes of IL-10 and TGF-β in peripheral blood were detected by double-antibody sandwich ELISA.

　　Results: Compared with the non-intervention group, the muscle pathological inflammatory cell infiltration of the mice in the intervention group was significantly reduced, and the levels of IL-10 and TGF-β in peripheral blood were significantly higher than those in the non-intervention group (P<0.05). The expressions of PD-1 and CTLA-4 were significantly increased (P<0.05).

　　Conclusion: The therapeutic effect of CD4+CD25+ Foxp3+ Treg cell reinfusion on EAM mice is exerted by increasing the levels of IL-10 and TGF-β in peripheral blood and the expression of PD-1 and CTLA-4 on the surface of CD4+ CD25+ Foxp3+ Treg cells in spleen. effect.