白色念珠菌,小鼠模型 z-bo 2020-08-12 590

　　(1) Replication method Use nude mice to carry out infection experiments of Candida albicans, Cryptococcus neoformans, histoplasma capsularis, and skin fungi, as well as experiments to observe the pathogenesis of psoriasis patients transplanted into the skin. ①A lethal infection model of Cryptococcus neoformans was established with BALB/c and Swiss nude mice. ②Inoculate BALB/c nude mice intraperitoneally with H. capsulatum, causing fatal infection, while heterozygous mice usually do not have fatal infection, and only a small amount of H. capsulatum multiplies and grows. ③The BALB/C (nu/nu) nude mice were selected as experimental subjects, and the Candida albicans and Cryptococcus suspensions were injected into the abdomen to establish a systemic infection model. The preparation concentration of the Candida albicans suspension was 1.6×10000000 CFU/ml. The concentration of Cryptococcus neoformans suspension was 2.0×10000000 CFU/ml.

　　(2) Characteristics of the model The main organs of this model animal were found to be infected with Cryptococcus neoformans or Candida albicans, which is relatively stable. Nude mice are animals with congenital immunodeficiency. They are used as experimental animals. There is no need to use cortisone acetate or cyclophosphamide to suppress the animal’s immunity during the experiment. The nude mice are prone to infection by fungi and bacteria. Fungal infection is easier in animal models. After nude mice are infected by fungi, their immunity is not easy to self-adjust and recover for a long period of time, so it is suitable for animal models with more complicated experimental design. Nude mice lack T cells, but they have normal B cells and NK cells. This is to explore how the humoral immune system, mainly B cells and NK cells, responds to the absence of T cell-based cellular immunity Fungal infection creates an excellent model.

　　(3) Comparative Medicine The establishment of this model provides a valuable experimental model for studying the physiological characteristics, immunogenicity, and pathogenesis of Cryptococcus neoformans and Candida albicans infection.