动物模型,帕金森 z-bo 2020-08-12 742

　　1. Modeling materials Animals: Lewis rats, male, weighing 260-280 g; medicine: rotenone.

　　2. Modeling method Rotenone (1.0g/L) was dissolved in a mixed solvent of dimethyl sulfoxide: normal saline (2:1). The normal control group was injected subcutaneously with a mixed solvent without rotenone, and the model group was injected subcutaneously with rotenone [1.0mg/ (kg·d)], divided into two injections, once at 8:00 in the morning and evening, for 30 days of continuous administration, and one day a week for stopping.

　　3. Principle of Modeling Rotenone has a selective damaging effect on rat SN dopamine neurons, and characteristic Lewy bodies can appear, but it has no effect on cholinergic neurons.

　　4. Changes after model building The weight of rats in the control group increased, and the rats in the model group lost weight first. As time passed, their weight gradually recovered to a certain level and remained unchanged, but was always lower than the control group.

　　5. Pathological and biochemical changes after modeling. Count under immunofluorescence microscope at 10 times. Compared with the control group, the number of TH-positive cells in the substantia nigra of the model group was significantly reduced, and the remaining neuron membranes and nuclear membranes were unclear.

　　Through Nissl staining, it can be seen under the microscope that the number of neurons in the substantia nigra of the model group has decreased, and the surviving neurons have shrunk, accompanied by the proliferation of glial cells.