动物造模 z-bio 2022-03-04 316

　　Bovine mastitis is the most common disease in dairy cows and the most expensive disease to treat. Numerous experiments have confirmed that micrornA (miRNA) plays an important role in regulating the innate and adaptive immune responses of mammals including cattle. In order to discover the expression changes of miRNAs and genes related to the NF-κB signaling pathway between healthy and mastitis-affected Chinese Holstein cows, Professor Liu Xiaolin from Northwest A&F University led the research group to carry out systematic experiments. The relevant research results were published in In the July issue of GENE.

　　The researchers first used small RNA sequencing technology (the high-throughput sequencing experiment was undertaken by Lianchuan Biotechnology) for miRNAome discovery, and then used qRT-PCR to analyze 25 miRNAs (miR-16 , miR-125b, miR-15, miR-29a, miR-23b, miR-146, miR-301a, miR-181b, let-7, miR-30b, miR-21, miR-223, miR-27b, miR -10a, miR-143, etc.) and 14 genes from nine tissues (RelA, RelB, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBε, IκBζ, Bcl-3, IKKα, IKKβ, IKKγ/NEMO ) for quantitative detection.

　　It was found that the expression levels of most miRNAs were decreased in dairy cows with mastitis, while the expression levels of RelA, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBζ, Bcl-3, and IKKα were increased .

　　Bovine mastitis is the most common disease in dairy cows and the most expensive disease to treat. Numerous experiments have confirmed that micrornA (miRNA) plays an important role in regulating the innate and adaptive immune responses of mammals including cattle. In order to discover the expression changes of miRNAs and genes related to the NF-κB signaling pathway between healthy and mastitis-affected Chinese Holstein cows, Professor Liu Xiaolin from Northwest A&F University led the research group to carry out systematic experiments. The relevant research results were published in In the July issue of GENE.

　　The researchers first used small RNA sequencing technology (the high-throughput sequencing experiment was undertaken by Lianchuan Biotechnology) for miRNAome discovery, and then used qRT-PCR to analyze 25 miRNAs (miR-16 , miR-125b, miR-15, miR-29a, miR-23b, miR-146, miR-301a, miR-181b, let-7, miR-30b, miR-21, miR-223, miR-27b, miR -10a, miR-143, etc.) and 14 genes from nine tissues (RelA, RelB, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBε, IκBζ, Bcl-3, IKKα, IKKβ, IKKγ/NEMO ) for quantitative detection.

　　It was found that the expression levels of most miRNAs were decreased in dairy cows with mastitis, while the expression levels of RelA, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBζ, Bcl-3, and IKKα were increased . Therefore, the researchers speculate that the activation of the NF-κB signaling pathway in individuals with mastitis is due to reduced miRNA inhibition. In contrast, the NF-κB signaling pathway is inactive in healthy individuals because miRNAs enhance repression.

　　This study provides new insights into the regulation of miRNAs on the NF-κB signaling pathway, and some differentially expressed miRNAs may become new markers for predicting mastitis in dairy cows.

　　Therefore, the researchers speculate that the activation of NF-κB signaling in individuals with mastitis is due to reduced miRNA inhibition. In contrast, the NF-κB signaling pathway is inactive in healthy individuals because miRNAs enhance repression.

　　This study provides new insights into the regulation of miRNAs on the NF-κB signaling pathway, and some differentially expressed miRNAs may become new markers for predicting mastitis in dairy cows.