动物造模,2型糖尿病 z-bio 2023-04-11 478

　　Objective Recent studies have shown that gut microbes are related to metabolic diseases such as type 2 diabetes mellitus (T2DM), and related mechanisms are mostly studied in rodents, but there are few reports on pig models that are more suitable for studying human diseases.

　　Methods In order to explore the changes in the composition and structure of gut microbes in the minipig T2DM model, the Guangxi Bama minipig was used as an animal model to induce T2DM through a high-fat and high-sugar diet. ) and the normal diet-fed control group (CN group) fresh fecal samples, 16S rRNA gene sequencing technology was used to compare the composition and structure of gut microbes.

　　Results The results showed that the diversity of gut microbes in the T2DM group decreased significantly; at the phylum level, compared with the CN group, the abundance of Firmicutes in the T2DM group was significantly increased (P<0.05), while the At the genus level, PCoA analysis found that the composition of CN group and T2DM group was significantly different. The genera with significant differences: Oscillaria, Prevotella and Peptococcus were significantly enriched in the CN group, and g_Intestinibacter was significantly enriched in the T2DM group; no metabolic pathways were found to be significantly different between the two groups in functional prediction. Both groups were mainly enriched in amino acid biosynthesis, carbon metabolism, amino sugar and nucleotide sugar metabolism, as well as glycolysis and gluconeogenesis.

　　Conclusion This study found the change characteristics of gut microbes in type 2 diabetic minipigs and potential microbes that are closely related to the occurrence of diabetes.