动物造模,基因敲除 z-bio 2022-07-01 473

　　Objective To breed and identify the genotype of Bmal1 knockout mice to provide an ideal animal model for biological rhythm research.

　　Methods The introduced Bmal1 knockout mice were reared and bred in a cage of 1 male and 2 females. The mouse tail genomic DNA was extracted from the offspring, the target gene fragments were amplified by PCR, and the gene results were determined by agar gel electrophoresis. The results were verified by Western Blot detection of Bmal1 protein expression in myocardial tissue.

　　Results Compared with Bmal1+/+ mice, Bmal1-/- mice showed no significant difference in phenotype except for body weight. Homozygous knockout mice lost their ability to reproduce; The knockout mice were successfully bred, and a batch of gene knockout mice was obtained.

　　Conclusion The PCR method can successfully identify the Bmall gene knockout homozygous mice, and the PCR amplification method is an effective method to detect the mouse gene.