动物造模 z-bio 2022-04-25 427

　　Objective To introduce an improved rat jugular vein cannula fabrication method and jugular vein cannulation operation method, and apply it to an experimental study of autonomous drug administration in rats to evaluate the service life and application effect of the cannula design.

　　METHODS: Twenty-nine male SD rats who had completed food training underwent jugular vein cannulation with modified cannula to establish a model of self-administration of propofol and cocaine, and the animals were eliminated 5 months after the operation.

　　Results Both the propofol self-administration model and the cocaine self-administration model were successfully established. The main reasons for the post-operative animal elimination were dying, tube blockage, and fluid leakage. From the 1st to 5th month after the operation, it was directly related to the intubation. The relevant elimination rates are 7%, 7%, 10%, 31%, and 11%, respectively. Under the condition that the monthly elimination rate is acceptable within 10%, the service life of the cannula assembly is expected to be at least 3 months. The service life of the cannula can be as long as 5 months or even more than 5 months. The cause of animal dying is related to severe liver and kidney damage, electrolyte imbalance and inflammation in the body.

　　Conclusion The improved cannula for autonomous drug administration in the jugular vein of rats has many advantages. The optimal service life of the cannula for rats with the intubation components designed in this paper can be as long as 3 months, which can meet the basic needs of autonomous drug administration experiments. , so the application effect is good and can be popularized.