动物造模,小鼠胚胎移植技术,巨细胞病毒 z-bio 2022-03-07 268

　　Objective To establish a purification method for cytomegalovirus in mice to obtain mice without MCMV infection.

　　Methods Using embryo transfer technology, the purification conditions were optimized by using different hormones, injecting hormones in different estrus cycles, recipient mouse strains, different transfer methods, embryo transfer at different stages, and the number of transferred embryos. Donor mice were decontaminated using an optimized embryo transfer decontamination method.

　　Results The hormone produced by SIGMA, the number of available embryos obtained by superovulation at a dose of 10 IU was about 17 per mouse; the most available embryos were obtained by superovulation in the interestrus of mice, and the number of available embryos by superovulation was about 23 per mouse. The offspring of C57 male mice mated with ICR female mice were selected as recipient mice, and the litter rate was 44.5%; fallopian tube transplantation was better than uterine transplantation, and the litter rate was 40.64%; the pregnancy rate of transplanted 2-cell embryos was 40.64%. 80%, significantly better than single-cell and 8-cell; when the recipient mice were transplanted with 24 embryos, the farrowing rate reached 43.75%. Using the optimized purification method of mouse cytomegalovirus embryo transfer, MCMV-free mice were purified.

　　Conclusion The purification method of mouse cytomegalovirus has been established, which provides a reliable guarantee for obtaining the mouse population without MCMV infection.