动物造模 z-bio 2022-03-08 365

　　Objective To provide theoretical and technical support for microbial detection of imported experimental monkeys and how to carry out feeding management and disease prevention and control by identifying and drug susceptibility testing of Proteus mirabilis isolated from the intestinal microbes of imported cynomolgus monkeys.

　　Methods The fecal samples of cynomolgus monkeys were collected from anal swabs, and bacterial isolation and culture, biochemical identification, drug sensitivity test and mouse pathogenicity test were carried out.

　　Results Eight of the 60 cynomolgus monkeys were isolated from suspicious bacteria, which were identified as Proteus mirabilis, indicating that the infection rate of Proteus mirabilis in this group of cynomolgus monkeys was 13.3%. , Cefoxime, cefoperazone, cefazolin, penicillin, etc. are highly sensitive, moderately sensitive to amikacin, gentamicin, ampicillin sodium, nitrofurantoin, etc., and low sensitive to norfloxacin, tetracycline, , resistance to bacitracin, ciprofloxacin, kanamycin, lincomycin and furazolidone; the mouse pathogenicity test showed that the isolated strain had strong pathogenicity to mice.

　　Conclusion The infection of Proteus mirabilis in cynomolgus monkeys is relatively high. The bacteria are sensitive to cephalosporin antibiotics and can be used as the first-choice drug for the treatment of the disease in clinical practice. The bacterium has strong pathogenicity and is potentially harmful to the cynomolgus monkey population, so it should be paid attention to in the usual feeding and management work.