动物造模 z-bio 2022-03-08 286

　　Objective Coxsackievirus A16 is one of the main pathogens of hand, foot and mouth disease, and its pathogenic mechanism is not yet clear. In this study, genetic diversity mice (Genetic Diversity Mice) were used to screen CA16 pathogenic related genes, and to further study the pathogenic mechanism of the virus.

　　Methods Twenty-three genetically diverse mouse strains were selected as the experimental group, and the inbred C57BL/6 mice were selected as the negative control group. All of them were infected with 107 Copies CA16 virus through the abdominal cavity. The viral load of the samples was analyzed, and the infection data were analyzed using The Gene Mine phylogenetic database to obtain CA16 pathogenic related genes.

　　Results According to the screening criteria that the number of virus copies in the blood and muscle of infected mice were higher than 104 Copies/ (μL/mg), seven genetically diverse mouse strains susceptible to CA16 were obtained, and the skeletal muscle tissue of these mice was It showed significant pathological damage; at the same time, based on the corresponding data analysis of The Gene Mine professional database, it was found that mouse chromosomes 3 and 4 were most related to CA16 pathogenesis, and finally 16 genes that might be related to CA16 pathogenesis were screened.

　　Conclusion The genetically diverse mouse resources provide new ideas for the study of CA16 pathogenic related genes.