动物实验,血脑屏障作用 z-bo 2020-08-13 476

　　[Fundamental]

　　The blood-brain barrier is composed of the pia mater, the capillary wall of the choroid plexus and the glial membrane formed by the glial cells outside the wall. It acts as a natural barrier and can block pathogenic microorganisms and toxic products, foreign particles including dye particles Wait from the bloodstream to enter the brain tissue and cerebrospinal fluid, thereby protecting the central nervous system from damage.

　　[Experimental Materials]

　　2 mice.

　　1ml sterile syringe (with 4 gauge needle).

　　5% trypan blue aqueous solution.

　　0.1ml of physiological saline.

　　Eye scissors and tweezers, etc.

　　[experimental method]

　　Use a 1ml sterile syringe to draw 5% trypan blue water solution into two mice through the tail vein, 0.7ml each, change the syringe, and inject 0.1ml of normal saline into one of the mice.

　　After 5-10 minutes, observe the color changes of the mouse skin, especially the eyes and mouth.

　　Within 30-60 minutes, the eyes and mouth of the mouse were seen blue, that is, died of suffocation, and the abdomen was fixed downward.

　　Cut the skin along the dorsal midline from head to tail to expose the subcutaneous, muscle and internal organs and observe the color change.

　　Carefully dissecting the skull and vertebrae, exposing the brain and spinal cord, compare with subcutaneous, muscle and internal organs; and compare the differences between the two mice.

　　[Experiment Results]

　　In mice without intracranial injection, the eyes, ears, nose, subcutaneous and muscles all showed obvious blue color, but the brain and spinal cord were not discolored; while the above parts of mice injected intracranially showed obvious blue.

　　[Precautions]

　　Trypan blue water solution can be used after filtering.

　　For tail vein injection, the needle should be inserted remotely; if the bolus is easy, it means that it has entered the blood vessel; if it causes subcutaneous bulging or whitening, it means that it has not entered the blood vessel; pull out the needle, and insert the needle from a short distance.