动物造模 z-bio 2022-03-08 507

　　Objective To compare the differences of peripheral blood and immune cell typing indexes between aged SD rats and young SD rats.

　　Methods 12-month-old and 8-week-old SD rats were subjected to the detection of peripheral blood cell count, white blood cell classification, immune organ coefficient, peripheral blood immune cell typing, spleen immune cell typing and splenic T cell P16 expression.

　　Results Compared with young rats, aged rats had decreased peripheral blood white blood cell count, percentage of lymphocytes, increased red blood cells, hemoglobin, percentage of neutrophils, percentage of eosinophils, and percentage of basophils, platelets and monocytes There was no difference in the percentage of cells, thymus coefficient, spleen coefficient, no difference between old rats and young rats, peripheral blood immune cell typing, old rat helper T cells, regulatory T cells, natural killer cells and monocytes The proportion of B cells increased and the proportion of B cells decreased. The results of spleen lymphocyte typing showed that the B cells (CD45RA) of the aged rats were lower than those of the young rats (P<0.01), and there was no difference in CD3 between the two groups. The expression of P16 mRNA in rat spleen T cells was significantly higher than that in young male rats (P<0.05).

　　Conclusion The types of peripheral blood cells and immune cells in aged rats are changed, and the expression of P16 in spleen T cells may be used as a biological marker of immune aging. The test results are compared and analyzed in this paper, which will provide basic data for the study of senile diseases and aging animal models.