动物造模,狂犬病毒 z-bio 2022-04-22 559

　　Objective: To establish a mouse anti-rabies virus IgG antibody ELISA method for the detection and analysis of rabies mouse model.

　　Methods: The optimal dilution of the sample and the optimal concentration of the secondary antibody were determined by orthogonal experiments; the specificity, sensitivity and stability of the method were analyzed; it was used for mouse samples together with commercial kits to determine the compliance rate of the ELISA method.

　　Results: The optimal sample dilution of this ELISA method was 1∶100, the optimal concentration of secondary antibody was 40 ng/mL, and the positive critical value was 0.121. The ELISA method did not cross-react with the positive serum of common mouse viruses; the lower limit of detection concentration was 129 μg/mL; the coefficient of variation of the three replicates of the sample is less than 10%. The coincidence rate with the commercial kit is 100%.

　　Conclusion: The ELISA detection method of mouse anti-rabies virus IgG antibody was successfully established, which can be applied to the analysis of rabies mouse model and the evaluation of vaccine titer.