动物造模,肠道菌群 z-bio 2022-08-10 393

　　OBJECTIVE: To detect the gut microbiota structure of tree shrew with high-grade sarcoma, and to provide a scientific basis for exploring the correlation between changes in gut microbiota structure and tumorigenesis.

　　Methods: The feces of 3 cases of tree shrew with malignant high-grade sarcoma were selected as the CA group, and the feces of 6 cases of healthy tree shrew were selected as the control NO group. The 16SV3+4 region primers were used to identify the bacterial diversity. Clustering, species annotation and abundance analysis were performed to obtain the gut microbial structure, abundance and diversity of the two groups of tree shrews.

　　RESULTS: Comparing the gut microbiome structure of tree shrew in CA group and NO group, there was no significant difference in the diversity index Chao1 index value, ACE index value and Shannon index value (P>0.05). The intestinal flora of healthy tree shrew was 640 species, compared with 494 species in the CA group. Compared at the phylum level, the abundance of Spirochaetes in the CA group was 0.03%, which was lower than that in the NO group (abundant phylum Spirochaetes). The abundance of Firmicutes in the CA group was 77.79% higher than that in the NO group (47.65%), but the difference was not statistically significant; the CA group The abundance of Bacteroidetes in the group was 0.75% lower than that in the NO group (8.31%), but the difference was not statistically significant. Lactobacillus in the CA group was a statistically significant biomarker.

　　Conclusion: The structural abundance and diversity of gut microbiota in tree shrew with high-grade sarcoma are lower than those in healthy tree shrew. The structural changes of gut microbiota and the increase of Firmicutes content may increase the occurrence of malignant tumors.