动物造模 z-bio 2022-05-31 398

　　Objective: To investigate the differences between BALB/c mutant curly hair mice and control BALB/c mice in the natural healing process of skin injury.

　　Methods: 10 6-week-old BALB/c mutant curly-haired mice and 10 control BALB/c mice were selected, and 2 round full-thickness skin wounds with a diameter of 0.5 cm were made on the back of the mice to prepare a skin injury model. On the 3rd, 7th, 10th, and 14th days after the injury, the healing of the skin shape was observed, the change of the skin damage area was measured, and the wound healing rate was calculated. , HE staining and Sirius red staining were performed to observe the morphological changes of the skin during the healing process.

　　Results: The appearance results showed that the wounds of the mutant curly-haired mice healed significantly faster at 3d and 7d after the wound. The results of the healing rate showed that the healing rate of the skin of the curly-haired mice at 3d and 7d after the wound was significantly higher than that of the control group. The healing rate of the mice was statistically significant (P<0.05), but there was no significant difference in the healing rate between the two groups of mice on the 10th and 14th days after the wound. The number of inflammatory cells infiltrated, capillaries were dilated and congested, collagen increased rapidly, granulation tissue increased significantly, and part of the epidermis regenerated and grew on 7 days after the wound. It is clearly seen that collagen fibers and granulation tissue gradually replace and repair the damaged parts from the bottom layer until the epidermis and hair follicles grow out.

　　Conclusion: The healing ability of BALB/c mutant curly-haired mice is stronger than that of control mice in the early stage after skin trauma, which provides a theoretical reference for the future application of the mutant mice to skin wound healing models.