动物造模 z-bio 2023-05-04 602

　　Objective: To compare and observe the differences of peripheral blood and immune system-related indexes between aged C57BL/6J mice and young C57BL/6J mice.

　　Methods: 19-month-old and 2-month-old C57BL/6J mice were collected to detect peripheral blood cell count, immune organ index and peripheral blood immune cell typing, respectively.

　　Results: Compared with young mice, the counts of red blood cells and platelets in peripheral blood of aged mice increased, the proportion of neutrophils increased, the proportion of lymphocytes decreased (P<0.001), and the proportion of CD4+ cells was significantly decreased (P<0.001). The thymus index was significantly decreased (P<0.001).

　　Conclusion: The differentiation of blood cells in aged mice is shifted, and the number of helper T cells is decreased, which is significantly different from that of young mice, and is similar to the changes in human hematopoietic immune aging. This study provides basic data for the study of immune aging-related issues.