动物造模 z-bio 2022-04-24 443

　　Objective: To study the effect of sodium fluoride on the histopathology of bone, brain, liver and kidney in rats.

　　Methods: Twenty healthy weaned SD rats, half male and half male, were randomly divided into control group and fluorosis group. Rats in fluorosis group drank tap water containing sodium fluoride (fluoride ion concentration: 100 mg/L) freely, and regularly After 12 weeks of feeding, the fluoride content in the femur, liver, kidney and brain tissue of the rats in each group was detected, and serum, liver, kidney and brain tissue were determined. Aldehyde (MDA) content, and the pathological structure changes of each tissue were observed under light microscope and electron microscope.

　　Results: The food intake, water intake, body weight, body length and tail length of the rats in the fluorosis group were slightly lower than those in the control group, but the difference was not statistically significant; the rats in the control group had no dental fluorosis, and the rats in the fluorosis group had fluorosis. The dental incidence rate was 100%; compared with the control group, the fluoride content of the femur, liver, kidney and brain tissue of the rats in the fluorosis group were significantly increased (P<0.01); the serum, liver, kidney and brain of the rats in the fluorosis group were significantly increased (P<0.01). The content of MDA in the tissue was higher than that in the control group (P<0.01 or P<0.05). The light microscope results showed that the thickness of the trabecular bone was uneven and the spacing increased in the fluorosis group; the hepatocyte was partially edema, and the arrangement of the hepatocyte cord was disordered. ; The proximal convoluted tubule epithelial cells of the renal cortex were swollen and the lumen was enlarged; the nerve cells in the hippocampus were swollen and vacuolized. Under the electron microscope, the osteoblasts, hepatocytes, renal proximal tubule epithelial cells and brain neurons of the rats in the fluorosis group were observed The ultrastructural features of the cytoplasmic cells were typical of apoptosis, including nuclear pyknosis, chromatin condensation, mitochondrial swelling, and vacuolization. Conclusion Sodium fluoride can induce experimental rat bone, liver, kidney and brain injury. Histopathological damage, leading to apoptosis.